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Article

Dissecting Genomic Aberrations in Myeloproliferative Neoplasms by Multiplex-PCR and Next Generation Sequencing.

PloS one - 1 Jan 2015

Kirschner Martin M J, Schemionek Mirle, Schubert Claudia, Chatain Nicolas, Sontag Stephanie, Isfort Susanne, Ortiz-Brüchle Nadina, Schmitt Karla, Krüger Luisa, Zerres Klaus, Zenke Martin, Brümmendorf Tim H, Koschmieder Steffen

Abstract excerpt

In order to assess the feasibility of amplicon-based parallel next generation sequencing (NGS) for the diagnosis of myeloproliferative neoplasms (MPN), we investigated multiplex-PCR of 212 amplicons covering genomic mutational hotspots in 48 cancer-related genes. Samples from 64 patients with MPN and five controls as well as seven (myeloid) cell lines were analyzed. Healthy donor and reactive erythrocytosis...

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Source

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Identifiers

PMID: 25894969
DOI: 10.1371/journal.pone.0123476
PMCID: PMC4404337

Genes

IDH1
JAK2
NRAS

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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