Article
Identification of peptide-specific TCR genes by in vitro peptide stimulation and CDR3 length polymorphism analysis.
Cancer letters - 10 Jul 2015
Shao Hongwei, Lin Yanmei, Wang Teng, Ou Yusheng, Shen Han, Tao Changli, Wu Fenglin, Zhang Wenfeng, Bo Huaben, Wang Hui, Huang Shulin
Abstract excerpt
Identification of TCR genes specific for tumor-associated antigens (TAAs) is necessary for TCR gene modification of T cells, which is applied in anti-tumor adoptive T cell therapy (ACT). The usual identification methods are based on isolating single peptide-responding T cells and cloning the TCR gene by in vitro expansion or by single-cell RT-PCR. However, the long and exacting in vitro culture period and...
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