Article
Ancestral amino acid substitution improves the thermal stability of recombinant lignin-peroxidase from white-rot fungi, Phanerochaete chrysosporium strain UAMH 3641.
Protein engineering, design & selection : PEDS - 1 Jul 2015
Semba Yasuyuki, Ishida Manabu, Yokobori Shin-ichi, Yamagishi Akihiko
Abstract excerpt
Stabilizing enzymes from mesophiles of industrial interest is one of the greatest challenges of protein engineering. The ancestral mutation method, which introduces inferred ancestral residues into a target enzyme, has previously been developed and used to improve the thermostability of thermophilic enzymes. In this report, we studied the ancestral mutation method to improve the chemical and thermal stabilities...
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