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Article

Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase.

The FEBS journal - 1 Jul 2015

Deszcz Dawid, Affaticati Pierre, Ladkau Nadine, Gegel Alex, Ward John M, Hailes Helen C, Dalby Paul A

Abstract excerpt

We have analyzed the natural evolution of transaminase structure and sequence between an α-transaminase serine-pyruvate aminotransferase and an ω-transaminase from Chromobacterium violaceum with < 20% sequence identity, and identified the active-site regions that are least conserved structurally. We also show that these structural changes correlate strongly with transaminase substrate specificity during evolution...

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PMID: 25846556
DOI: 10.1111/febs.13293

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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