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Improving Hydrolytic Activity and Enantioselectivity of Epoxide Hydrolase from Phanerochaete chrysosporium by Directed Evolution.

Molecules (Basel, Switzerland) - 14 Oct 2024

Shao Huanhuan, Xu Pan, Tao Xiang, He Xinyi, Pu Chunyan, Liang Shaorong, Shi Yingxin, Wang Xiaoyan, Feng Hong, Yong Bin

Abstract excerpt

Epoxide hydrolases (EHs) catalyze the conversion of epoxides into vicinal diols. The epoxide hydrolase gene from P. chrysosporium was previously cloned and subjected to site-directed mutation to study its enzyme activity, but the results were unsatisfactory. This study used error prone PCR and DNA shuffling to construct a PchEHA mutation library. We performed mutation-site combinations on PchEHA based on enzyme...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39459231
DOI: 10.3390/molecules29204864
PMCID: PMC11509930

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