Article
Development of an in vivo gene mutation assay using the endogenous Pig-A gene: II. Selection of Pig-A mutant rat spleen T-cells with proaerolysin and sequencing Pig-A cDNA from the mutants.
Environmental and molecular mutagenesis - 1 Oct 2008
Miura Daishiro, Dobrovolsky Vasily N, Mittelstaedt Roberta A, Kasahara Yoshinori, Katsuura Yasuhiro, Heflich Robert H
Abstract excerpt
We previously reported that rat spleen T-cells and peripheral red blood cells that are deficient in glycosylphosphatidylinositol (GPI) synthesis [presumed mutants for the phosphatidylinositol glycan complementation group A gene (Pig-A)] could be detected by flow cytometry (FCM) as cells negative for GPI-linked markers (CD48 and CD59, respectively). To establish this procedure as a rapid in vivo gene mutation...
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