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Analysis of mutations in the Pig-a gene of spleen T-cells from N-ethyl-N-nitrosourea-treated fisher 344 rats.

Environmental and molecular mutagenesis - 1 Jun 2011

Miura Daishiro, Shaddock Joseph G, Mittelstaedt Roberta A, Dobrovolsky Vasily N, Kimoto Takafumi, Kasahara Yoshinori, Heflich Robert H

Abstract excerpt

A rapid in vivo somatic cell gene mutation assay is being developed that measures mutation in the endogenous X-linked phosphatidylinositol glycan, class A gene (Pig-a). The assay detects Pig-a mutants by flow cytometric identification of cells deficient in glycosylphosphatidyl inositol (GPI) anchor synthesis. GPI-deficient, presumed Pig-a mutant cells also can be detected in a cloning assay that uses proaerolysin...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 21542029
DOI: 10.1002/em.20654

Genes

GPI

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Analysis of mutations in the Pig-a gene of spleen T-cells from N-ethyl-N-nitrosourea-treated fisher 344 rats.PMID 21542029Read ↗

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