Article
Identifying a Rab effector on the macroautophagy pathway.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Wang Juan, Cervantes Serena, Davis Saralin, Ferro-Novick Susan
Abstract excerpt
Rab GTPases are key regulators of membrane traffic. The Rab GTPase Ypt1 is essential for endoplasmic reticulum (ER)-Golgi traffic, intra-Golgi traffic, and the macroautophagy pathway. To identify effectors on the macroautophagy pathway, known autophagy-related genes (Atg genes) required for macroautophagy were tagged with GFP and screened for mislocalization in the ypt1-2 mutant. At the pre-autophagosomal...
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