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MIP-MAP: High-Throughput Mapping of Caenorhabditis elegans Temperature-Sensitive Mutants via Molecular Inversion Probes.

Genetics - 1 Oct 2017

Mok Calvin A, Au Vinci, Thompson Owen A, Edgley Mark L, Gevirtzman Louis, Yochem John, Lowry Joshua, Memar Nadin, Wallenfang Matthew R, Rasoloson Dominique, Bowerman Bruce, Schnabel Ralf, Seydoux Geraldine, Moerman Donald G, Waterston Robert H

Abstract excerpt

Mutants remain a powerful means for dissecting gene function in model organisms such as Caenorhabditis elegans Massively parallel sequencing has simplified the detection of variants after mutagenesis but determining precisely which change is responsible for phenotypic perturbation remains a key step. Genetic mapping paradigms in C. elegans rely on bulk segregant populations produced by crosses with the...

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PMID: 28827289
DOI: 10.1534/genetics.117.300179
PMCID: PMC5629315

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MIP

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