Article
TILLING mutants of durum wheat result in a high amylose phenotype and provide information on alternative splicing mechanisms.
Plant science : an international journal of experimental plant biology - 1 Apr 2015
Sestili Francesco, Palombieri Samuela, Botticella Ermelinda, Mantovani Paola, Bovina Riccardo, Lafiandra Domenico
Abstract excerpt
The amylose/amylopectin ratio has a major influence over the properties of starch and determines its optimal end use. Here, high amylose durum wheat has been bred by combining knock down alleles at the two homoelogous genes encoding starch branching enzyme IIa (SBEIIa-A and SBEIIa-B). The complete silencing of these genes had a number of pleiotropic effects on starch synthesis: it affected the transcriptional...
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