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Article

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.

Chemical communications (Cambridge, England) - 21 Mar 2015

Bar-Shir Amnon, Liang Yajie, Chan Kannie W Y, Gilad Assaf A, Bulte Jeff W M

Abstract excerpt

Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

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Identifiers

PMID: 25697683
DOI: 10.1039/c4cc10195b
PMCID: PMC4485562

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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