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Article

Multiplex detection of functional G protein-coupled receptors harboring site-specifically modified unnatural amino acids.

Biochemistry - 27 Jan 2015

Naganathan Saranga, Ray-Saha Sarmistha, Park Minyoung, Tian He, Sakmar Thomas P, Huber Thomas

Abstract excerpt

We developed a strategy for identifying positions in G protein-coupled receptors that are amenable to bioorthogonal modification with a peptide epitope tag under cell culturing conditions. We introduced the unnatural amino acid p-azido-l-phenylalanine (azF) into human CC chemokine receptor 5 (CCR5) at site-specific amber codon mutations. We then used strain-promoted azide-alkyne [3+2] cycloaddition to label the...

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Source

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Identifiers

PMID: 25524496
DOI: 10.1021/bi501267x
PMCID: PMC4310623

Genes

CCR5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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