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Article

Tracking of Engineered Bacteria In Vivo Using Nonstandard Amino Acid Incorporation.

ACS synthetic biology - 15 Jun 2018

Praveschotinunt Pichet, Dorval Courchesne Noémie-Manuelle, den Hartog Ilona, Lu Chaochen, Kim Jessica J, Nguyen Peter Q, Joshi Neel S

Abstract excerpt

The rapidly growing field of microbiome research presents a need for better methods of monitoring gut microbes in vivo with high spatial and temporal resolution. We report a method of tracking microbes in vivo within the gastrointestinal tract by programming them to incorporate nonstandard amino acids (NSAA) and labeling them via click chemistry. Using established machinery constituting an orthogonal translation...

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Identifiers

PMID: 29791796
DOI: 10.1021/acssynbio.8b00135
PMCID: PMC6415965

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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