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Article

Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.

Biochemistry - 4 Mar 2014

Ray-Saha Sarmistha, Huber Thomas, Sakmar Thomas P

Abstract excerpt

We developed a strategy for creating epitope maps of monoclonal antibodies (mAbs) that bind to G protein-coupled receptors (GPCRs) containing photo-cross-linkers. Using human CXC chemokine receptor 4 (CXCR4) as a model system, we genetically incorporated the photolabile unnatural amino acid p-azido-l-phenylalanine (azF) at various positions within extracellular loop 2 (EC2). We then mapped the interactions of the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24490954
DOI: 10.1021/bi401289p
PMCID: PMC3985944

Genes

CXCR4
CCR5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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