Article
One-step molecular detection of the MYD88 L265P mutation by unlabeled probe genotyping analysis.
Molecular and cellular probes - 1 Feb 2015
Poon Kok-Siong, Liu Te-Chih, Tan Karen Mei-Ling, Koay Evelyn Siew-Chuan
Abstract excerpt
The aim of our study was to establish an unlabeled probe genotyping approach for rapid detection of the MYD88 L265P mutation in the differential diagnosis of Waldenstrӧm macroglobulinemia patients. Analytical and clinical validation of the assay was performed using serially diluted amplicon-cloned standards, 14 clinical bone marrow aspirate samples, and 30 peripheral blood samples from healthy donors,...
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