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Article

Setting up a probe based, closed tube real-time PCR assay for focused detection of variable sequence alterations.

Pathology oncology research : POR - 1 Oct 2014

Becságh Péter, Szakács Orsolya

Abstract excerpt

During diagnostic workflow when detecting sequence alterations, sometimes it is important to design an algorithm that includes screening and direct tests in combination. Normally the use of direct test, which is mainly sequencing, is limited. There is an increased need for effective screening tests, with "closed tube" during the whole process and therefore decreasing the risk of PCR product contamination. The aim...

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PMID: 24659043
DOI: 10.1007/s12253-014-9759-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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