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Article

A simple method for 3D analysis of immunolabeled axonal tracts in a transparent nervous system.

Cell reports - 20 Nov 2014

Belle Morgane, Godefroy David, Dominici Chloé, Heitz-Marchaland Céline, Zelina Pavol, Hellal Farida, Bradke Frank, Chédotal Alain

Abstract excerpt

Clearing techniques have been developed to transparentize mouse brains, thereby preserving 3D structure, but their complexity has limited their use. Here, we show that immunolabeling of axonal tracts followed by optical clearing with solvents (3DISCO) and light-sheet microscopy reveals brain connectivity in mouse embryos and postnatal brains. We show that the Robo3 receptor is selectively expressed by medial...

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Source

Identifiers

PMID: 25456121
DOI: 10.1016/j.celrep.2014.10.037

Genes

DCC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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