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Direct analysis of mAb aggregates in mammalian cell culture supernatant

28 Nov 2014

Abstract excerpt

BACKGROUND: Protein aggregation during monoclonal antibody (mAb) production can occur in upstream and downstream processing (DSP). Current methods to determine aggregate formation during cell culture include size exclusion chromatography (SEC) with a previous affinity chromatography step in order to remove disturbing cell culture components. The pre-purification step itself can already influence protein...

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PMID: 25431119
DOI: 10.1186/s12896-014-0099-3

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