Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Synonymous rare arginine codons and tRNA abundance affect protein production and quality of TEV protease variant.

PloS one - 1 Jan 2014

Fang Jie, Zou Lingling, Zhou Xuan, Cheng Beijiu, Fan Jun

Abstract excerpt

It has been identified that a TEV protease (TEVp) variant, TEVp(5M), displays improved solubility. Here, we constructed fifteen TEVp(5M) variants with one or more of six rare arginine codons in the coding sequence replaced with abundant E. coli arginine codons. These codon variants expressed in either E. coli BL21 (DE3) or Rossetta (DE3) showed different solubility and activity. Supply of rare tRNAs increased the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arginine
Share to Endopeptidases
Share to Genetic Engineering

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Isoenzymes

Share to Open Reading Frames

Share to Protein Biosynthesis

Share to RNA, Transfer

Share to Solubility

Source

Open in PubMed Open full text

Identifiers

PMID: 25426854
DOI: 10.1371/journal.pone.0112254
PMCID: PMC4245098

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Synonymous rare arginine codons and tRNA abundance affect protein production and quality of TEV protease variant.PMID 25426854Read ↗

Combination of the mutations for improving activity of TEV protease in inclusion bodies.Semantic proximity Read ↗

In vivo and in vitro characterization of TEV protease mutants.Semantic proximity Read ↗

Construction, Investigation and Application of TEV Protease Variants with Improved Oxidative Stability.Semantic proximity Read ↗

P1' specificity of the S219V/R203G mutant tobacco etch virus protease.Semantic proximity Read ↗

A tobacco etch virus protease with increased substrate tolerance at the P1' position.Semantic proximity Read ↗

Tobacco etch virus (TEV) protease with multiple mutations to improve solubility and reduce self-cleavage exhibits enhanced enzymatic activity.Semantic proximity Read ↗

Engineering soluble tobacco etch virus protease accompanies the loss of stability.Semantic proximity Read ↗

Application of the wheat-germ cell-free translation system to produce high temperature requirement A3 (HtrA3) proteases.Semantic proximity Read ↗

Improved solubility of TEV protease by directed evolutionSemantic proximity Read ↗

Effect of tandem rare codon substitution and vector-host combinations on the expression of the EBV gp110 C-terminal domain in Escherichia coli.Semantic proximity Read ↗

An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents.Semantic proximity Read ↗

Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.