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Article

In vivo and in vitro characterization of TEV protease mutants.

Protein expression and purification - 1 Jun 2012

Wei Lingling, Cai Xueyan, Qi Zhenguo, Rong Liang, Cheng Beijiu, Fan Jun

Abstract excerpt

Tobacco etch virus protease (TEVp) is frequently applied in the cleavage of fusion protein. However, production of TEV protease in Escherichia coli is hampered by low yield and poor solubility, and auto-cleavage of wild type TEVp gives rise to the loss-of-function. Previously it was reported that TEVp S219V displayed more stability, and TEVp variant containing T17S/N68D/I77V and double mutant L56V/S135G resulted...

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Identifiers

PMID: 22484199
DOI: 10.1016/j.pep.2012.03.011

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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