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Article

Application of the wheat-germ cell-free translation system to produce high temperature requirement A3 (HtrA3) proteases.

BioTechniques - 1 Jan 2012

Singh Harmeet, Makino Shin-ichi, Endo Yaeta, Li Ying, Stephens Andrew N, Nie Guiying

Abstract excerpt

Mammalian high temperature requirement A3 (HtrA3) is a serine protease of the HtrA family. It is an important factor for placental development and a tumor suppressor. The biochemical properties of HtrA3 are uncharacterized. One critical step in biochemical characterization is overexpressing and purifying the full-length recombinant protein. However, utility of cell-based expression systems is limited for a...

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PMID: 22229724
DOI: 10.2144/000113798

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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