Article
The efficacy of uracil DNA glycosylase pretreatment in amplicon-based massively parallel sequencing with DNA extracted from archived formalin-fixed paraffin-embedded esophageal cancer tissues.
Cancer genetics - 1 Sept 2015
Serizawa Masakuni, Yokota Tomoya, Hosokawa Ayumu, Kusafuka Kimihide, Sugiyama Toshiro, Tsubosa Yasuhiro, Yasui Hirofumi, Nakajima Takashi, Koh Yasuhiro
Abstract excerpt
Advances in mutation testing for molecular-targeted cancer therapies have led to the increased use of archived formalin-fixed paraffin-embedded (FFPE) tumors. However, DNA extracted from FFPE tumors (FFPE DNA) is problematic for mutation testing, especially for amplicon-based massively parallel sequencing (MPS), owing to DNA fragmentation and artificial C:G > T:A single nucleotide variants (SNVs) caused by...
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