Article
Immunohistochemical detection of the BRAF V600E mutation in melanoma patients with monoclonal antibody VE1.
Pathology international - 1 Dec 2014
Liu Hui, Li Zhongwu, Wang Yan, Feng Qin, Si Lu, Cui Chuanliang, Guo Jun, Xue Weicheng
Abstract excerpt
A novel mutation-specific monoclonal antibody VE1 was generated to detect BRAF V600E mutation with immunohistochemistry. This study aims to investigate the sensitivity and specificity of immunohistochemistry compared with conventional Sanger sequencing and to evaluate whether IHC would become the routine screening method of BRAF V600E mutation. A total of 84 cases of melanoma lesion specimens were selected to...
Topics
- Adult
- Antibodies, Monoclonal
- Biomarkers, Tumor
- DNA Mutational Analysis
- Female
- Humans
- Immunohistochemistry
- Male
- Melanoma
- Middle Aged
- Mutation