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Article

Clinical massively parallel next-generation sequencing analysis of 409 cancer-related genes for mutations and copy number variations in solid tumours.

British journal of cancer - 11 Nov 2014

Singh R R, Patel K P, Routbort M J, Aldape K, Lu X, Manekia J, Abraham R, Reddy N G, Barkoh B A, Veliyathu J, Medeiros L J, Luthra R

Abstract excerpt

BACKGROUND: In a clinical diagnostic laboratory, we evaluated the applicability of the Ion Proton sequencer for screening 409 cancer-related genes in solid tumours. METHODS: DNA was extracted from formalin-fixed, paraffin-embedded (FFPE) tissue biopsy specimens of 55 solid tumours (20 with matched normal tissue) and four cell lines and screened for mutations in 409 genes using the Ion Proton system. The mutation...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25314059
DOI: 10.1038/bjc.2014.518
PMCID: PMC4229640

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MIP
ALK
APC
MET
PTEN

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