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Tracking cancer drugs in living cells by thermal profiling of the proteome

2 Oct 2014

Abstract excerpt

The thermal stability of proteins can be used to assess ligand binding in living cells. We have generalized this concept by determining the thermal profiles of more than 7000 proteins in human cells by means of mass spectrometry. Monitoring the effects of small-molecule ligands on the profiles delineated more than 50 targets for the kinase inhibitor staurosporine. We identified the heme biosynthesis enzyme...

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PMID: 25278616
DOI: 10.1126/science.1255784

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