Article
Structural insights into activation of the retinal L-type Ca²⁺ channel (Cav1.4) by Ca²⁺-binding protein 4 (CaBP4).
The Journal of biological chemistry - 7 Nov 2014
Park Saebomi, Li Congmin, Haeseleer Françoise, Palczewski Krzysztof, Ames James B
Abstract excerpt
CaBP4 modulates Ca(2+)-dependent activity of L-type voltage-gated Ca(2+) channels (Cav1.4) in retinal photoreceptor cells. Mg(2+) binds to the first and third EF-hands (EF1 and EF3), and Ca(2+) binds to EF1, EF3, and EF4 of CaBP4. Here we present NMR structures of CaBP4 in both Mg(2+)-bound and Ca(2+)-bound states and model the CaBP4 structural interaction with Cav1.4. CaBP4 contains an unstructured N-terminal...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Calcium
- Calcium Channels
- Calcium Channels, L-Type
- Calcium-Binding Proteins
- Calmodulin
- Calorimetry
- Humans
- Magnesium
- Magnetic Resonance Spectroscopy
- Mice
- Models, Molecular