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Article

Expression and purification of human FANCI and FANCD2 using Escherichia coli cells.

Protein expression and purification - 1 Nov 2014

Takahashi Daisuke, Sato Koichi, Shimomuki Mayo, Takata Minoru, Kurumizaka Hitoshi

Abstract excerpt

The DNA interstrand crosslink (ICL) is an extremely deleterious DNA lesion that covalently crosslinks complementary strands and prevents the strand-separation reaction. In higher eukaryotes, the Fanconi anemia proteins, FANCI and FANCD2, form a heterodimer and play essential roles in ICL repair. Human FANCI and FANCD2 are large proteins with molecular masses of 149kDa and 164kDa, respectively, and were reportedly...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 25168188
DOI: 10.1016/j.pep.2014.08.012

Genes

FANCD2
FANCI

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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