Article
Full-length myocilin protein is purified from mammalian cells as a dimer.
Protein expression and purification - 1 Jul 2018
Katoli Parvaneh, Godbole Adarsh, Romanowski Michael J, Clark Kirk, Meredith Erik, Saenz-Vash Veronica, Wang Y Karen, Lewicki Nancy, Nguyen Andrew A, Lynch Jeffrey M
Abstract excerpt
Myocilin (MYOC) is a secreted protein found in human aqueous humor (AH) and mutations in the MYOC gene are the most common mutation observed in glaucoma patients. Human AH analyzed under non-reducing conditions suggests that MYOC is not normally found in a monomeric form, but rather is predominantly dimeric. Although MYOC was first reported almost 20 years ago, a technical challenge still faced by researchers is...
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