Article
[Analysis of ambiguities in HLA sequencing-based typing and its solutions].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Feb 2015
Wang Daming, He Liumei, Zou Hongyan, Gao Suqing, Deng Zhihui
Abstract excerpt
OBJECTIVE: To investigate the number and ratio of ambiguous allele combinations from human leukocyte antigen (HLA) confirmatory test by sequencing based typing for unrelated donor marrow transplantation, and to establish an efficient strategy for identifying such ambiguities. METHODS: A total of 650 donor-receipt samples were genotyped for 5 loci of the HLA gene using an Atria SBT commercial kit. Exons 2, 3 and 4...
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