Article
CRISPR-mediated direct mutation of cancer genes in the mouse liver.
Nature - 16 Oct 2014
Xue Wen, Chen Sidi, Yin Hao, Tammela Tuomas, Papagiannakopoulos Thales, Joshi Nikhil S, Cai Wenxin, Yang Gillian, Bronson Roderick, Crowley Denise G, Zhang Feng, Anderson Daniel G, Sharp Phillip A, Jacks Tyler
Abstract excerpt
The study of cancer genes in mouse models has traditionally relied on genetically-engineered strains made via transgenesis or gene targeting in embryonic stem cells. Here we describe a new method of cancer model generation using the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system in vivo in wild-type mice. We used hydrodynamic injection to deliver a CRISPR...
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