Novel NAT2 haplotyping using allele-specific sequencing.

Abstract excerpt

BACKGROUND: NAT2 is a common metabolizer of many clinical drugs. NAT2 haplotyping requires a complex procedure. Allele-specific PCR followed by direct sequencing or cloning sequencing are common methods used for haplotyping. However, these common methods require labor-intensive procedures. Allele-specific sequencing was designed for haplotyping of the NAT2 gene. MATERIALS & METHODS: Using rapid DNA polymerase...

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