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Article

Redesigning the blue copper azurin into a redox-active mononuclear nonheme iron protein: preparation and study of Fe(II)-M121E azurin.

Journal of the American Chemical Society - 3 Sept 2014

Liu Jing, Meier Katlyn K, Tian Shiliang, Zhang Jun-Long, Guo Hongchao, Schulz Charles E, Robinson Howard, Nilges Mark J, Münck Eckard, Lu Yi

Abstract excerpt

Much progress has been made in designing heme and dinuclear nonheme iron enzymes. In contrast, engineering mononuclear nonheme iron enzymes is lagging, even though these enzymes belong to a large class that catalyzes quite diverse reactions. Herein we report spectroscopic and X-ray crystallographic studies of Fe(II)-M121E azurin (Az), by replacing the axial Met121 and Cu(II) in wild-type azurin (wtAz) with Glu...

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PMID: 25082811
DOI: 10.1021/ja505410u

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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