Article
The mutation Met121-->His creates a type-1.5 copper site in Alcaligenes denitrificans azurin.
European journal of biochemistry - 1 Sept 1996
Kroes S J, Hoitink C W, Andrew C R, Ai J, Sanders-Loehr J, Messerschmidt A, Hagen W R, Canters G W
Abstract excerpt
The Cu ligand Met121 in azurin of Alcaligenes denitrificans was mutated to His. The spectroscopic and mechanistic properties of [M121H]azurin appear to be pH dependent with a pKa of 3.8 due to the ionization of His121. The [M121H]azurin mutant exhibits two major distinct metal-site-coordination g...
Topics
- Alcaligenes
- Azurin
- Binding Sites
- Cloning, Molecular
- Copper
- Electrochemistry
- Electron Spin Resonance Spectroscopy
- Escherichia coli
- Gene Expression
- Histidine
- Hydrogen-Ion Concentration
- Ligands
- Magnetic Resonance Spectroscopy
- Methionine
- Mutagenesis, Site-Directed
- Mutation
- Spectrum Analysis
- Spectrum Analysis, Raman