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Article

Improved stability and half-life of fluorinated phosphotriesterase using Rosetta.

Chembiochem : a European journal of chemical biology - 18 Aug 2014

Yang Ching-Yao, Renfrew P Douglas, Olsen Andrew J, Zhang Michelle, Yuvienco Carlo, Bonneau Richard, Montclare Jin Kim

Abstract excerpt

Recently we demonstrated that incorporating p-fluorophenylalanine (pFF) into phosphotriesterase dramatically improved folding, thereby leading to enhanced stability and function at elevated temperatures. To further improve the stability of the fluorinated enzyme, Rosetta was used to identify multiple potential stabilizing mutations. One such variant, pFF-F104A, exhibited enhanced activity at elevated temperature...

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PMID: 25066940
DOI: 10.1002/cbic.201402062

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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