Short-term exposure to fluconazole induces chromosome loss in Candida albicans: an approach to produce haploid cells.

doi:10.1016/j.fgb.2014.06.009

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Short-term exposure to fluconazole induces chromosome loss in Candida albicans: an approach to produce haploid cells.

Fungal genetics and biology : FG & B - 1 Sept 2014

Chang Fang-Mo, Ou Tsong-Yih, Cheng Wei-Ning, Chou Ming-Li, Lee Kai-Cheng, Chin Yi-Ping, Lin Chih-Peng, Chang Kai-Di, Lin Che-Tong, Su Ching-Hua

Abstract excerpt

Candida albicans is considered to be an obligate diploid fungus. Here, we describe an approach to isolate aneuploids or haploids induced by the short-term (12-16 h) exposure of diploid reference strains SC5314 and CAI4 to the most commonly used antifungal drug, fluconazole, followed by repeated single-cell separation among small morphologically distinct colonies in the inhibition zone. The isolated strains had...

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Short-term exposure to fluconazole induces chromosome loss in Candida albicans: an approach to produce haploid cells.

Abstract excerpt

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Short-term exposure to fluconazole induces chromosome loss in Candida albicans: an approach to produce haploid cells.

Abstract excerpt

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