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Article

New examples of membrane protein expression and purification using the yeast based Pdr1-3 expression strategy.

Journal of biotechnology - 10 Dec 2014

Gupta Rakeshkumar P, Kueppers Petra, Schmitt Lutz

Abstract excerpt

Overexpression and purification of membrane proteins has been a bottleneck for their functional and structural study for a long time. Both homologous and heterologous expression of membrane proteins with suitable tags for purification presents unique challenges for cloning and expression. Saccharomyces cerevisiae is a potential host system with significant closeness to higher eukaryotes and provides opportunity...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25036752
DOI: 10.1016/j.jbiotec.2014.07.010

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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