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Article

Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae.

Biochemical and biophysical research communications - 11 Jul 2008

Ito Keisuke, Sugawara Taishi, Shiroishi Mitsunori, Tokuda Natsuko, Kurokawa Azusa, Misaka Takumi, Makyio Hisayoshi, Yurugi-Kobayashi Takami, Shimamura Tatsuro, Nomura Norimichi, Murata Takeshi, Abe Keiko, Iwata So, Kobayashi Takuya

Abstract excerpt

Crystallization of eukaryotic membrane proteins is a challenging, iterative process. The protein of interest is often modified in an attempt to improve crystallization and diffraction results. To accelerate this process, we took advantage of a GFP-fusion yeast expression system that uses PCR to d...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 18474222
DOI: 10.1016/j.bbrc.2008.04.182

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-18474222-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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