Article
Cell-cycle synchrony for analysis of S. pombe DNA replication.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Luche Douglas D, Forsburg Susan L
Abstract excerpt
Analysis of S phase events requires the ability to synchronize the cell cycle for subsequent physiological or molecular analysis. The cell cycle can be arrested at different stages, using drugs or temperature-sensitive mutations, and then released (block and release). These methods offer high levels of synchrony. Synchrony is lower in methods that fractionate the cells (lactose gradient synchrony), which avoids...
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