Article

Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples

2014-01-01

Abstract excerpt

We have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. Our method uses targeted RNase H-based digestion to remove contaminating poly(rA) carrier and ribosomal RNA. This depletion step improves both the quality of data and quantity of informative reads in unbiased total RNA sequenc...

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Identifiers and source

Literature Corpus work: e6ad639c-54ce-5f81-b145-6fd4286438e4
DOI: 10.1186/preaccept-1698056557139770

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