Article
[Development of a method for the separation of HLA-A, -B and -C haploid using biotinylated probe and streptavidin magnetic beads].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Jun 2014
Tao Sudan, He Yanmin, Zhang Wei, Wang Wei, He Ji, Zhu Famin, Lyu Hangjun
Abstract excerpt
OBJECTIVE: To develop a method for separating the human leukocyte antigen (HLA)-A, -B and -C haploid using biotinylated probes and streptavidin magnetic beads in order to solve ambiguous HLA genotyping results. METHODS: Based on sequence information of HLA alleles from the IMGT/HLA database, the 5-biotinylated probes were designed. The probe was mixed and extended with corresponding genomic DNA, and incubated...
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