Article
Selection of dinB alleles suppressing survival loss upon dinB overexpression in Escherichia coli.
Journal of bacteriology - 15 Aug 2014
Benson Ryan W, Cafarelli Tiziana M, Rands Thomas J, Lin Ida, Godoy Veronica G
Abstract excerpt
Escherichia coli strains overproducing DinB undergo survival loss; however, the mechanisms regulating this phenotype are poorly understood. Here we report a genetic selection revealing DinB residues essential to effect this loss-of-survival phenotype. The selection uses strains carrying both an antimutator allele of DNA polymerase III (Pol III) α-subunit (dnaE915) and either chromosomal or plasmid-borne dinB...
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