Article
Single-molecule kinetics of the eukaryotic initiation factor 4AI upon RNA unwinding.
Structure (London, England : 1993) - 8 Jul 2014
Sun Yingjie, Atas Evrim, Lindqvist Lisa M, Sonenberg Nahum, Pelletier Jerry, Meller Amit
Abstract excerpt
The eukaryotic translation initiation factor 4AI (eIF4AI) is the prototypical DEAD-box RNA helicase. It has a "dumbbell" structure consisting of two domains connected by a flexible linker. Previous studies demonstrated that eIF4AI, in conjunction with eIF4H, bind to loop structures and repetitively unwind RNA hairpins. Here, we probe the conformational dynamics of eIF4AI in real time using single-molecule FRET....
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