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Article

Identification of constitutively active AtMPK6 mutants using a functional screen in Saccharomyces cerevisiae.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Hudik Elodie, Berriri Souha, Hirt Heribert, Colcombet Jean

Abstract excerpt

MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed protocol for their screening, taking advantage of a functional...

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PMID: 24908120
DOI: 10.1007/978-1-4939-0922-3_6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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