Article
Structural and mutagenic analysis of the RM controller protein C.Esp1396I.
PloS one - 1 Jan 2014
Martin Richard N A, McGeehan John E, Kneale Geoff
Abstract excerpt
Bacterial restriction-modification (RM) systems are comprised of two complementary enzymatic activities that prevent the establishment of foreign DNA in a bacterial cell: DNA methylation and DNA restriction. These two activities are tightly regulated to prevent over-methylation or auto-restriction. Many Type II RM systems employ a controller (C) protein as a transcriptional regulator for the endonuclease gene...
Topics
- Crystallography, X-Ray
- DNA
- DNA Restriction Enzymes
- Models, Molecular
- Mutation
- Protein Conformation
- Surface Plasmon Resonance