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Article

A phenotypic high throughput screening assay for the identification of pharmacoperones for the gonadotropin releasing hormone receptor.

Assay and drug development technologies - 1 May 2014

Conn P Michael, Smith Emery, Spicer Timothy, Chase Peter, Scampavia Louis, Janovick Jo Ann

Abstract excerpt

We describe a phenotypic high throughput screening (HTS) calcium flux assay designed to identify pharmacoperones for the gonadotropin releasing hormone receptor (GnRHR). Pharmacoperones are target-specific, small molecules that diffuse into cells, rescue misfolded protein mutants, and restore them to function. Rescue is based on correcting the trafficking of mutants that would otherwise be retained in the...

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Identifiers

PMID: 24831790
DOI: 10.1089/adt.2014.576
PMCID: PMC4025569

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