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Article

Double-strand break repair and genetic recombination in topoisomerase and primase mutants of bacteriophage T4.

DNA repair - 1 Sept 2014

Shcherbakov Victor P, Kudryashova Elena

Abstract excerpt

The effects of primase and topoisomerase II deficiency on the double-strand break (DSB) repair and genetic recombination in bacteriophage T4 were studied in vivo using focused recombination. Site-specific DSBs were induced by SegC endonuclease in the rIIB gene of one of the parents. The frequency/distance relationship was determined in crosses of the wild-type phage, topoisomerase II mutant amN116 (gene 39), and...

Topics

Bacteriophage T4
DNA Primase
DNA Topoisomerases
Mutation
Recombination, Genetic
Recombinational DNA Repair
Viral Proteins

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 24811919
DOI: 10.1016/j.dnarep.2014.04.009

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