Article
Double-strand break repair and recombination-dependent replication of DNA in bacteriophage T4 in the absence of UvsX recombinase: replicative resolution pathway.
DNA repair - 1 May 2012
Shcherbakov Victor P, Plugina Lidia, Shcherbakova Tamara, Kudryashova Elena, Sizova Svetlana
Abstract excerpt
The effects of mutations in bacteriophage T4 genes uvsX and 49 on the double-strand break (DSB)-promoted recombination were studied in crosses, in which DSBs were induced site-specifically within the rIIB gene by SegC endonuclease in the DNA of only one of the parents. Frequency of rII+ recombinants was measured in two-factor crosses of the type i×ets1 and in three-factor crosses of the type i×ets1 a6, where ets1...
Topics
- Bacteriophage T4
- Crosses, Genetic
- DNA Breaks, Double-Stranded
- DNA Repair
- DNA Replication
- DNA-Binding Proteins
- Gene Order
- Membrane Proteins
- Models, Genetic
- Mutation
- Rec A Recombinases
- Recombination, Genetic
- Viral Proteins