Article
Viewing single lambda site-specific recombination events from start to finish.
The EMBO journal - 4 Oct 2006
Mumm Jeffrey P, Landy Arthur, Gelles Jeff
Abstract excerpt
The site-specific recombination pathway by which the bacteriophage lambda chromosome is excised from its Escherichia coli host chromosome is a tightly regulated, highly directional, multistep reaction that is executed by a series of multiprotein complexes. Until now, it has been difficult to study the individual steps of such reactions in the context of the entire pathway. Using single-molecule light microscopy,...
Topics
- Attachment Sites, Microbiological
- Bacteriophage lambda
- DNA, Bacterial
- DNA, Viral
- Escherichia coli
- Integrases
- Kinetics
- Mutation
- Recombination, Genetic