Pyrosequencing evaluation of low-frequency KRAS mutant alleles for EGF receptor therapy selection in metastatic colorectal carcinoma.

doi:10.2217/fon.13.233

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

Pyrosequencing evaluation of low-frequency KRAS mutant alleles for EGF receptor therapy selection in metastatic colorectal carcinoma.

Future oncology (London, England) - 1 Apr 2014

Natalicchio Maria Iole, Improta Giuseppina, Zupa Angela, Cursio Olga Elisabetta, Stampone Emanuela, Possidente Luciana, Teresa Gerardi Assunta Maria, Vita Giulia, Martini Maurizio, Cassano Alessandra, Piccoli Claudia, Romito Sante, Aieta Michele, Antonetti Raffaele, Barone Carlo, Landriscina Matteo

Abstract excerpt

AIM: To evaluate whether pyrosequencing (PS) improves the KRAS mutational status predictive value. PATIENTS & METHODS: A retrospective analysis of KRAS mutations by PS and direct sequencing (DS) in 192 metastatic colorectal carcinomas (mCRCs), subgrouped in 51 KRAS mutated at PS and 141 KRAS wild-type at DS. RESULTS: DS failed to detect low-frequency KRAS mutations in four out of 51 mCRCs, whereas PS detected 12...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antibodies, Monoclonal, Humanized

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Colorectal Neoplasms

Share to Disease-Free Survival

Share to ErbB Receptors

Share to Neoplasm Metastasis

Share to Proto-Oncogene Proteins

Share to Proto-Oncogene Proteins p21(ras)

Share to ras Proteins

Source

Open in PubMed

Identifiers

PMID: 24799053
DOI: 10.2217/fon.13.233

Genes

KRAS
EGFR
EGF
BRAF
NRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Pyrosequencing evaluation of low-frequency KRAS mutant alleles for EGF receptor therapy selection in metastatic colorectal carcinoma.PMID 24799053Read ↗

Effect of low-frequency KRAS mutations on the response to anti-EGFR therapy in metastatic colorectal cancer.Semantic proximity Read ↗

KRAS analysis in colorectal carcinoma: analytical aspects of Pyrosequencing and allele-specific PCR in clinical practice.Semantic proximity Read ↗

Rare RAS Mutations in Metastatic Colorectal Cancer Detected During Routine RAS Genotyping Using Next Generation Sequencing.Semantic proximity Read ↗

KRAS gene mutation in a series of unselected colorectal carcinoma patients with prognostic morphological correlations: a pyrosequencing method improved by nested PCR.Semantic proximity Read ↗

Development of pyrosequencing methods for the rapid detection of RAS mutations in clinical samples.Semantic proximity Read ↗

KRAS, BRAF, and TP53 deep sequencing for colorectal carcinoma patient diagnostics.Semantic proximity Read ↗

Optimization of RAS/BRAF Mutational Analysis Confirms Improvement in Patient Selection for Clinical Benefit to Anti-EGFR Treatment in Metastatic Colorectal Cancer.Semantic proximity Read ↗

KRAS and BRAF mutations in 203 esophageal squamous cell carcinomas: pyrosequencing technology and literature review.Semantic proximity Read ↗

Challenges posed to pathologists in the detection of KRAS mutations in colorectal cancers.Semantic proximity Read ↗

Pyrosequencing-based methods reveal marked inter-individual differences in oncogene mutation burden in human colorectal tumours.Semantic proximity Read ↗

RAS-expanded Mutations and HER2 Expression in Metastatic Colorectal Cancer: A New Step of Precision Medicine.Semantic proximity Read ↗

454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine KRAS mutation analysis of formalin-fixed, paraffin-embedded samplesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.