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Insertion of core <scp>CpG</scp> island element into human <scp>CMV</scp> promoter for enhancing recombinant protein expression stability in <scp>CHO</scp> cells

30 Apr 2014

Abstract excerpt

The human cytomegalovirus promoter (hCMV) is susceptible to gene silencing in CHO cells, most likely due to epigenetic events, such as DNA methylation and histone modifications. The core CpG island element (IE) from the hamster adenine phosphoribosyltransferase gene has been shown to prevent DNA methylation. A set of modified hCMV promoters was developed by inserting one or two copies of IE in either forward or...

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PMID: 24789630
DOI: 10.1002/btpr.1919

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